ABSTRACT
@# Objective: To explore the action mechanism of miR-139-5p inhibiting proliferation and invasion of epithelial ovarian cancer (EOC) cells by targetedly regulatingNotch1.Methods: A total of 24 pairs of EOC tissues and its corresponding para-cancerous tissues from patients, who underwent surgical resection in the DepartmentofGynecology,Nanyang Central Hospital of Henan Province, were collected for this study; in addition, human ovarian cancer cell lines (SKOV3, ES2, HEY-T30) and human ovarian epithelial cell line IOSE80 were also collected. Real-time quantitative PCR (qPCR) was applied to detectmRNAexpressionofmiR-139-5pandNotch1 in EOC tissues and cell lines. The miR-139-5p over-expression vector and recombinant plasmid pLV-Notch1 were transfected into SKOV3 cells. Blank control group (Ctrl group) and negative control group (NC group) were set up. Dual luciferase reporter gene assay was applied to verify the targeting relationship between miR-139-5p and Notch1 3'-UTR. CCK-8, Transwell and Scratch healing experiments were applied to detect cell proliferationinvasionandmigration, respectively. Western blotting was applied to detect expressions of proliferation and migration related proteins in cells. Results: Compared with para-cancerous tissues and IOSE80 cells, the expression of miR-139-5p was significantly decreased in EOC tissues and cell lines, while the expression of Notch1 mRNA was significantly increased (all P<0.01). The results of Dual luciferase reporter showed that Notch1 was the downstream target gene of miR-139-5p. Compared with NC group, cell proliferation, invasion and migration ability, expression levels of Notch1, NICD, Cyclin D1, Cyclin A1, Snail1, β-catenin and N-cadherin were all significantly decreased on 3 d in miR-139-5p mimic group (all P<0.01), while expression of E-cadherin was significantly increased (P<0.01); meanwhile, over-expression of Notch1 could reverse the inhibitory effect of miR-1395p on proliferation, invasion and migration of SKOV3 cells. Conclusion: miR-139-5p can targetedly regulate Notch1 to inhibit proliferation, invasion and migration of EOC cells, which may be related to its down-regulation of NICD, Cyclin D1, Cyclin A1, Snail1, βcatenin and N-cadherin, and up-regulation of E-cadherin.
ABSTRACT
Objective@#To investigate the impacts of sports game curriculum on the physical development of preschool children aged 3 to 5 years, so as to provide a reference for sports education and intervention among preschool children.@*Methods@#Totally 176 preschool children aged 3 to 5 years in the large, medium and small class of a kindergarten in Changzhou were selected by means of cluster random sampling, and they were randomly divided into observation group (n=85) and control group(n=91). The control group did not receive special intervention, while the observation group received sports game curriculum, the intervention time was 1 year. The body shape and physical fitness indicators were tested and compared before and after intervention.@*Results@#In terms of body shape, the increase in height of the observation group was (6.57±0.41)cm, which was significantly higher than (5.77±0.36)cm of the control group(t=13.78, P<0.01); There was no significant difference in weight gain between the observation group and the control group (2.53±0.35)kg vs (2.61±0.43)kg(t=1.35, P>0.05). The physical fitness indicators, including standing long jump, continuous jumping of the feet, sit-and-reach, tennis throwing, single foot balance and 10 m×2 shuttle run effects were (95.93±4.02)cm, (6.72±0.35)s, (11.65±1.33)cm, (6.21±1.50)m, (45.26±8.74)s,(6.91±0.13)s in the observation group, significantly better than (82.71±3.59)cm, (7.52±0.41)s, (10.06±1.32)cm, (5.34±1.21)m, (36.14±6.72)s, (7.36±0.17)s in the control group(t=23.04, 13.88, 7.96, 4.25, 7.79, 19.63, P<0.05).@*Conclusion@#The sports game curriculum has no significant effect on the weight of preschool children aged 3 to 5, but it can promote the height growth of children and helps to improve physical fitness.
ABSTRACT
Anisodus acutangulus hairy roots were grown in N6 medium which was optimal for growth and alkaloid production. The cell biomass and alkaloid yield reached 2.7 g l-1 and 3.9 mg l-1 (dry weight) respectively in sucrose medium, higher than those obtained in other carbon source media. 90 mM nitrogen (NH4 +/NO3 – = 4:1) gave the highest cell yield (4.5 g l-1) and the maximum alkaloid production (9.9 mg l-1). The cell yield (4.1 g l-1) of hairy roots grown at pH 6.5 was 2 times higher than that at pH 4.5. However, the maximum alkaloid production (7.2 mg l-1) was yielded at pH 4.5.